Anti-ADAM10 Antibody Picoband®-iFluor647 Conjugate

Anti-ADAM10 Antibody Picoband®-iFluor647 Conjugate

• Background: A Disintegrin and metalloproteinase domain-containing protein 10, also known as ADAM10 or CDw156 or CD156c is a protein that in humans is encoded by the ADAM10 gene. Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.
• Description: Boster Bio Anti-ADAM10 Antibody Picoband® catalog # A00566-5. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Disintegrin and metalloproteinase domain-containing protein 10; ADAM 10; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD156c; ADAM10; KUZ; MADM
• Gene Name: ADAM metallopeptidase domain 10
• Gene ID: 102
• UniProt: O14672
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human ADAM10 recombinant protein (Position: A217-D311).
• Clonality: Polyclonal
• Applications: WB,Flow Cytometry,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283). Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) (PubMed:26686862, PubMed:11786905). Contributes to the normal cleavage of the cellular prion protein (PubMed:11477090). Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity (PubMed:12475894). Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity). Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form (PubMed:17557115). Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B (PubMed:19114711, PubMed:21288900). May regulate the EFNA5-EPHA3 signaling (PubMed:16239146).
• References & Citations: 1. Abel, S., Hundhausen, C., Mentlein, R., Schulte, A., Berkhout, T. A., Broadway, N., Hartmann, D., Sedlacek, R., Dietrich, S., Muetze, B., Schuster, B., Kallen, K.-J., Saftig, P., Rose-John, S., Ludwig, A. The transmembrane CXC-chemokine ligand 16 is induced by IFN-gamma and TNF-alpha and shed by the activity of the disintegrin-like metalloproteinase ADAM10. J. Immun. 172: 6362-6372, 2004. 2. Asai, M., Hattori, C., Szabo, B., Sasagawa, N., Maruyama, K., Tanuma, S., Ishiura, S. Putative function of ADAM9, ADAM10, and ADAM17 as APP alpha-secretase. Biochem. Biophys. Res. Commun. 301: 231-235, 2003. 3. Atapattu, L., Saha, N., Chheang, C., Eissman, M. F., Xu, K., Vail, M. E., Hii, L., Llerena, C., Liu, Z., Horvay, K., Abud, H. E., Kusebauch, U., and 9 others. An activated form of ADAM10 is tumor selective and regulates cancer stem-like cells and tumor growth. J. Exp. Med. 213: 1741-1757, 2016.
• Storage Conditions: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Observed Molecular Weight: 70 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.25-0.5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -