Recombinant Human CCL2, biotinylated

Recombinant Human CCL2, biotinylated

• Description: Recombinant human CCL2
• Product Name Alternative: rHuCCL2-biotin, MCP-1
• CAS Number: 9000-83-3
• Gene Name: CCL2
• UniProt: P13500
• Cellular Locus: Secreted
• Reactivity: Human
• Target Antigen: C-C motif chemokine 2
• Target: CCL2ylated
• Type: Protein
• Applications: Migration assay
• Dilution: Centrifuge tube prior to resuspending. Recommended at 100ug/ml in sterile distilled water.
• Form: Lyophilized
• Function: Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:9837883, PubMed:10587439, PubMed:10529171). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:9837883, PubMed:10587439). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8627182, PubMed:9792674, PubMed:8195247). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690). {PubMed:10529171, PubMed:10587439, PubMed:8107690, PubMed:8195247, PubMed:8627182, PubMed:9792674, PubMed:9837883}.
• Additionnal Information: Endotoxin Level: <0.01 EU per 1ug of protein by LAL method.Migration Assay of recombinant CCR2-expressing cells.
• Storage Conditions: 12 months from date of receipt, -20°C to -70°C, as supplied. 1 month,- 20°C to -70°C, under sterile conditions after reconstitution. Best if used immediately after reconstitution. Avoid multiple freeze-thaw cycles.
• Specificity: CCL2ylated
• Target Background: CCL2, also known as Monocyte Chemoattractant Protein-1 (MCP-1) is a small cytokine (~13kDa) that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to sites of inflammation produced by either tissue injury or infection. It is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. To become activated, CCL2 protein has to be cleaved by metalloproteinase MMP-12. CCL2 is implicated in the pathogenesis of several diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis. CCR2 and CCR4 are two cell surface receptors that bind CCL2.