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NANOG Antibody

CAT: 0013-GTR18124392Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124392Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NANOG Antibody
Product Name Alternative
NANOG, Nanog homeobox, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
UniProt
Q9H9S0
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-DLFLNYSMNMQPED
Target
NANOG
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1) . Recommended concentration: 0.05-0.1μg/mL. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources.Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2μg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant NANOG captured by a rabbit antibody. Recommended reporter concentration: 5-10μg/mL. This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB06860.
Tested Applications
EIA, ELISA, IHC, WB
NCBI Accession Number
NP_079141.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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