SNX26 Antibody

SNX26 Antibody

SNX26, TCGAP, FLJ39019, sorting nexin 26, likely ortholog of mouse TC10/CDC42 GTPase-activating protein, NOMA-GAP, neurite outgrowth multiadaptor RhoGAP protein

O14559

Human

The immunogen for this antibody is: SWSLHSEGQTRSYC

SNX26

Unconjugated

Signal Transduction

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

500 ug/mL

Liquid

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

For research use only.

Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 75kDa band in Human Bone Marrow, Kidney, and Pancreas lysates after 1μg/mL antibody staining. EB05916 may detect the isoform A1A5D2 (UniProtKB) with a molecular weight of 81.4kDa which is similar in amino acid sequence to NP_443180.2 however lacking aa729-1268. Please note that currently we cannot find a precise explanation in the literature for the band we observe given the calculated size of 121kDa according to NP_443180.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Brain shows cytoplasm staining of Purkinje cells in Cerebellum. Recommended concentration, 2.5μg/mL.

Mouse

ELISA, IHC

NP_443180.2

Goat