FGF-2 (basic) heat stable, 154 aa

• Description :

  FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic exerts proliferative activity towards a wide range of cell types and consequently is used as a vital component in numerous cell culture media formulations, including stem cell media. FGF-basic loses its activity over time in solution at physiological temperatures and needs to be resupplemented periodically to maintain its performance. As a result of selected amino acid substitutions, Heat Stable FGF-basic retains full biological activity and also maintains full activity over extended periods of time in solution at 37°C. These properties make Heat Stable FGF-basic a superior choice for use in cell culture media applications. Heat Stable Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acids.

• Synonyms :

  Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin

• NCBI Gene ID :

  2247

• UniProt :

  P09038

• Accession Number :

  NP_001997.5

• Accession Number mRNA :

  NM_002006.4

• Chromosomal Location :

  4q26

• Reactivity :

  Human

• Cross Reactivity :

  Human

• Sequence :

  AAGSITTLPA LPEDGGSGAF PPGHFKDPKL LYCKNGGFFL RIHPDGRVDG TRDKSDPFIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL YAIKNVTDEC FFFERLEENN YNTYRSRKYP SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute in 5mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  ≥ 95% by SDS-PAGE gel and HPLC analyses.

• Bioactivity :

  Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is 0.05-0.25 ng/ml.

• Length :

  154

• Form :

  Lyophilized

• Buffer :

  5mM Tris, pH 7.6 + 150mM NaCl

• Reconstitution :

  5mM Tris, pH 7.6

• Molecular Weight :

  17.2 kDa

• Storage Conditions :

  The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.

• Host or Source :

  E. coli