FGF-2 (basic) 146 aa

• Description:

  FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant Human FGF-basic (146 a.a.) is a 16.4 kDa protein consisting of 146 amino acid residues.

• Synonyms:

  FGF2; BFGF; FGFB; HBGF-2; basic Fibroblast growth factor (bFGF) ; Heparin binding growth factor-3, Prostatropin

• NCBI Gene ID:

  2247

• UniProt:

  P09038

• Accession Number:

  NP_001997.5

• Accession Number mRNA:

  NM_002006.4

• Chromosomal Location:

  4q26

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  PALPEDGGSG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH IKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTD ECFFFERLES NNYNTYRSRK YTSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in 5mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

• Length:

  146

• Form:

  Lyophilized

• Buffer:

  5mM Tris, pH 7.6 + 150mM NaCl

• Reconstitution:

  5mM Tris, pH 7.6

• Molecular Weight:

  16.4 kDa

• Storage Conditions:

  The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

• Host or Source:

  E. coli