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Double stranded RNA control Poly(I:C)

CAT: 0579-10090100Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0579-10090100Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The low molecular weight Poly(I:C) dsRNA sequence is intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements._x000D_ _x000D_ Positive control for dsRNA detection by anti dsRNA monoclonal J2, J5, K1 and K2 antibodies._x000D_ _x000D_ Caution: The Poly(I:C) dsRNA positive control, to be combined with our monoclonal anti dsRNA antibodies J2, J5, K1 and K2 in both the direct AN-ELISA and sandwich ELISA, is not intended to be used as a quantitative standard for other dsRNA preparations. The anti dsRNA antibodies may exhibit a different degree of reactivity with different dsRNAs obtained from natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out, for example the OD450, in the 4-parameter analysis. It cannot be used to determine the concentration of a different type of dsRNA. The only proper standard for each specific application is the purified dsRNA under investigation.
Synonyms
Double stranded RNA (dsRNA) Control/Poly(I):Poly(C)
Type
Control Lysate
Source
In vitro synthesized RNA (heteropolymer consisting of unmodified ribonucleotides)
Applications
ELISA, Dot blot
Field of Research
Infectious Agents and Disease, Microbiology
Assay Principle
Double stranded RNA control Poly(I:C) is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels. We sµggest to use it as a homogenous, well-characterized positive control for different dsRNA detection methods, e.g. for ELISA and dot-blots._x000D_ _x000D_ The optimum working dilution of the Poly(I:C) dsRNA control for any specific application should be established by titration.
Stability
Shelf Life; 24 months
Form
Each vial contains 50 μl of a 1 mg/mL Poy(I:C) solution in RNAse/DNAse-free STE Buffer (0,1 M NaCl, 1 mM EDTA, 50 mM Tris-HCl, pH 7,0). Low molecµLar weight polyinosine-polycytidylic acid (i.e. poly(I:C)) is a synthetic analog of double-stranded RNA (dsRNA) and is comprised of short strands of inosine poly(I) homopolymer annealed to strands of cytidine poly(C) homopolymer; The average size of Poly(I:C) LMW is 0,2 kb to 1 kb.
Shipping Conditions
Blue Ice
Storage Conditions
Store at -20 °C to -80 °C upon arrival, avoid freeze/thaw cycles, store in aliquots
Functional Analysis
Direct ELISA and Sandwich ELISA (relative activity to reference antigen)

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