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CD7 Mouse Monoclonal Antibody

CAT: 0013-GTR18111635-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18111635-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD7 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Native purified human CD7. Purification: The antibody was purified by affinity chromatography.
Product Name Alternative
T-cell antigen CD7; GP40; T-cell leukemia antigen; T-cell surface antigen Leu-9; TP41; CD antigen CD7
UniProt
P09564
Reactivity
Human
Immunogen
Native purified human CD7.
Target
CD7
Clonality
Monoclonal
Clone
BV5X5C3
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
The antibody was purified by affinity chromatography.
Dilution
IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .
Tested Applications
FC, IF
Host or Source
Mouse
Preservative
PBS, pH 7.3, and 0.02% sodium azide.
Isotype
Mouse IgG1
Entrez
924

UniProtKB · P09564

T-cell antigen CD7

CD7_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09564
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CD7
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Adaptive immunityImmunity

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

Cellular component

Membrane

Technical term

Proteomics identificationReference proteome

Molecular function

Receptor

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