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CD45 Mouse Monoclonal Antibody

CAT: 0013-GTR18111527-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18111527-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD45 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Native purified human CD45. Purification: The antibody was purified by affinity chromatography.
Product Name Alternative
CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD antigen CD45
UniProt
P08575
Reactivity
Human
Immunogen
Native purified human CD45.
Target
PTPRC
Clonality
Monoclonal
Clone
B1X6
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
The antibody was purified by affinity chromatography.
Dilution
IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .
Tested Applications
FC, IF
Host or Source
Mouse
Preservative
PBS, pH 7.3, and 0.02% sodium azide.
Isotype
Mouse IgG2a
Entrez
5788

UniProtKB · P08575

Receptor-type tyrosine-protein phosphatase C

PTPRC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08575
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PTPRC
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.1.3.48
Processing
Precursor
Secondary accessions
A0A0A0MT22, A8K7W6, Q16614, Q9H0Y6, X6R433
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSynapse

Disease

Disease variantSCID

PTM

GlycoproteinPhosphoprotein

Biological process

Host-virus interaction

Molecular function

HydrolaseProtein phosphatase

Domain

RepeatSignalTransmembraneTransmembrane helix

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