CD147 Mouse Monoclonal Antibody

CAT: 0013-GTR18111492-01Size: 30 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18111492-01Size:30 µL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

The CD147 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Native purified human CD147. Purification: The antibody was purified by affinity chromatography.

Product Name Alternative

Basigin; 5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; EMMPRIN; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF; CD antigen CD147

UniProt

P35613

Reactivity

Human

Immunogen

Native purified human CD147.

Target

BSG

Clonality

Monoclonal

Clone

BJKO8

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation; Cell Biology

Purification

The antibody was purified by affinity chromatography.

Dilution

IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .

Tested Applications

FC, IF

Host or Source

Mouse

Preservative

PBS, pH 7.3, and 0.02% sodium azide.

Isotype

Mouse IgG1

Entrez

682

UniProtKB · P35613

Basigin

BASI_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P35613
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: BSG
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: A6NJW1, D3YLG5, Q7Z796, Q8IZL7

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative promoter usageAlternative splicing

Biological process

AngiogenesisHost-virus interaction

Molecular function

Blood group antigenHost cell receptor for virus entryReceptor

Cellular component

Cell membraneCell projectionEndoplasmic reticulumEndosomeMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

Immunoglobulin domainSignalTransmembraneTransmembrane helix

Ligand

LectinMannose-binding

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