Recombinant Mouse Alpha Crystallin B

Recombinant Mouse Alpha Crystallin B

• Description: Recombinant Mouse Alpha Crystallin B
• Product Name Alternative: CRYAB Mouse
• CAS Number: 9000-83-3
• Gene Name: Cryab
• UniProt: P23927
• Cellular Locus: Cytoplasm, cytosol , Nucleus , Secreted , Lysosome , Note=Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. Localizes at the Z-bands and the intercalated disk in cardiomyocytes. Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion.
• Reactivity: Mouse
• Target Antigen: Alpha Crystallin B
• Target: Alpha Crystallin B
• Type: Protein
• Applications: WB
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Form: Liquid
• Buffer: 20 mM Tris
• Function: May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (PubMed:31786107). {PubMed:31786107}.
• Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store at or below -20°C for longer periods of time. For long term storage, addition of a carrier protein (0.1% HSA or BSA) is recommended.
• Specificity: Alpha Crystallin B
• Formulation: Sterile-filtered colorless solution contains 20mM Tris buffer, pH 8.0 and 10% glycerol.
• Buffer pH: pH 8.0
• Target Background: Alpha crystallins are composed of two gene products: alpha-A (acidic) and alpha-B (basic). Alpha crystallins are induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones. In addition, alpha crystallins have autokinase activity and play a role in intracellular architecture. Alpha crystallin B is expressed in many tissues and is associated with many neurological diseases.