DirectPCR Lysis Reagent (Ear)

CAT: 0388-401-ESize: 25 mLDry Ice: NoHazardous: No

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CAT#:0388-401-ESize:25 mL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

DirectPCR Lysis Reagent (Ear) is a molecular biology reagent for rapid DNA preparation from mouse ear tissue for direct PCR and genotyping. The single-tube DirectPCR® workflow enables preparation of PCR-compatible crude DNA lysates while reducing conventional DNA extraction and sample transfer steps. What is DirectPCR Lysis Reagent (Ear) | Mouse DNA PCR Genotyping? DirectPCR Lysis Reagent (Ear) is designed for the preparation of DNA from mouse ear pieces and ear-punch samples prior to PCR-based genotyping. The reagent combines tissue lysis with a formulation intended to reduce the inhibitory effects of crude biological lysates on PCR, allowing the resulting lysate to be used as a DNA template in compatible PCR workflows. Key Features → Designed for mouse ear tissue and ear-punch samples. → Suitable for direct PCR and genotyping. → Single-tube DNA preparation workflow. → Reduces sample transfer and DNA purification steps. → Designed to produce PCR-compatible crude DNA lysates. → No organic extraction reagents required. → 25 mL format for approximately 250 mouse ear pieces.

Applications

PCR Genotyping → Preparation of DNA templates from mouse ear and ear-punch samples for genomic PCR genotyping.
Mouse Genetic Screening → Supports screening of genetically engineered mouse colonies and identification of genotypes from ear-punch tissue.
Molecular Biology Research → Suitable for research workflows requiring rapid preparation of DNA from mouse tissue for downstream PCR analysis.

Research Use : DirectPCR lysis-based approaches have been used in published research for mouse ear tissue lysis and PCR-based genotyping, supporting the application of rapid crude DNA preparation in mouse genetic screening workflows.

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FAQ

What is DirectPCR Lysis Reagent (Ear)?

=> DirectPCR Lysis Reagent (Ear) is a PCR lysis reagent designed to prepare DNA from mouse ear tissue for direct PCR and genotyping.

What samples can be used with DirectPCR Ear Reagent?

=> The reagent is designed for mouse ear pieces and ear-punch tissue used in PCR-based genotyping workflows.

Can DirectPCR Ear Reagent be used for PCR genotyping?

=> Yes. The resulting crude DNA lysate is designed for use as a template in compatible genomic PCR genotyping workflows.

Does DirectPCR Ear Reagent include Proteinase K?

=> No. Proteinase K is required for the manufacturer's ear-tissue protocol but is not included with the DirectPCR Lysis Reagent.

What is the capacity of the 25 mL format?

=> The 0388-401-E 25 mL format is the smaller DirectPCR Ear reagent configuration. If the manufacturer has not explicitly specified the number of ear pieces for this size, I recommend not stating a numerical capacity on the product page.

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