CFSE Cell Division Tracker Kit

CAT: 0763-E-CK-A345-01Size: 500 AssaysDry Ice: NoHazardous: No
CAT#:0763-E-CK-A345-01Size:500 Assays
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Detection Method
Fluorometric method
Assay Principle
CFDA SE is a kind of fluorescein dye with membrane permeability, which has no fluorescence itself. When CFDA SE enters living cells through the cell membrane, it can be catalyzed by esterase in cytoplasm to produce carboxyfluorescein succinimide ester (CFSE), which can emit strong green fluorescence, can not penetrate the cell membrane, and can remain intact in the cell. CFSE can also spontaneously and irreversibly combine with amino groups of intracellular proteins to couple to these proteins. At the same time, excessive and uncoupled CFDA SE is passively diffused back into extracellular medium and removed by subsequent washing steps.
Sample Type
Cell samples
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Citation 01
https://www.nature.com/articles/s43018-025-00997-0
Detection Instrument
Flow Cytometer; Fluorescence Microscope
Product Datasheet
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