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MTT Cell Proliferation and Cytotoxicity Assay Kit

CAT: 0763-E-CK-A341-01Size: 500 AssaysDry Ice: NoHazardous: No
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CAT#:0763-E-CK-A341-01Size:500 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Detection Method
Colorimetric method
Assay Principle
MTT is 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide which can be reduced by some dehydrogenases in mitochondria to form a crystalline dark purple product formazan. Formazan can be completely dissolved by DMSO and its absorbance is near 570 nm wavelength. The more and faster the cells proliferate, the darker the color is, and the more cytotoxic, the lighter the color is. For the same cells, there is a linear relationship between the depth darkness of color and the number of cells.
Sample Type
Cell samples
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2-8°C for 12 months with shading light.
Citation 01
https://pubs.acs.org/doi/abs/10.1021/acsnano.4c07851
Citation 02
https://www.sciencedirect.com/science/article/pii/S0956566324007413
Citation 03
https://www.sciencedirect.com/science/article/pii/S0021979724029254
Citation 04
https://link.springer.com/article/10.1186/s13287-022-03114-5
Citation 05
https://link.springer.com/article/10.1007/s00395-025-01100-5
Citation 06
https://www.sciencedirect.com/science/article/pii/S0141813024045471
Citation 07
https://link.springer.com/article/10.1186/s12935-024-03302-8
Citation 08
https://www.mdpi.com/2072-6694/16/4/714
Citation 09
https://pubs.rsc.org/en/content/articlelanding/2025/en/d5en00071h
Citation 10
https://journals.lww.com/nrronline/abstract/9900/physical_exercise_promotes_white_matter_repair.783.aspx
Citation 11
https://www.sciencedirect.com/science/article/pii/S2352554125000804
Citation 12
https://www.mdpi.com/2073-4360/17/5/693
Detection Instrument
Microplate reader
Product Datasheet
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