MTT Cell Proliferation and Cytotoxicity Assay Kit

• Detection Method:

  Colorimetric method

• Assay Principle:

  MTT is 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide which can be reduced by some dehydrogenases in mitochondria to form a crystalline dark purple product formazan. Formazan can be completely dissolved by DMSO and its absorbance is near 570 nm wavelength. The more and faster the cells proliferate, the darker the color is, and the more cytotoxic, the lighter the color is. For the same cells, there is a linear relationship between the depth darkness of color and the number of cells.

• Sample Type:

  Cell samples

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2-8°C for 12 months with shading light.

• Citation 01:

  https://pubs.acs.org/doi/abs/10.1021/acsnano.4c07851

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S0956566324007413

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S0021979724029254

• Citation 04:

  https://link.springer.com/article/10.1186/s13287-022-03114-5

• Citation 05:

  https://link.springer.com/article/10.1007/s00395-025-01100-5

• Citation 06:

  https://www.sciencedirect.com/science/article/pii/S0141813024045471

• Citation 07:

  https://link.springer.com/article/10.1186/s12935-024-03302-8

• Citation 08:

  https://www.mdpi.com/2072-6694/16/4/714

• Citation 09:

  https://pubs.rsc.org/en/content/articlelanding/2025/en/d5en00071h

• Citation 10:

  https://journals.lww.com/nrronline/abstract/9900/physical_exercise_promotes_white_matter_repair.783.aspx

• Citation 11:

  https://www.sciencedirect.com/science/article/pii/S2352554125000804

• Citation 12:

  https://www.mdpi.com/2073-4360/17/5/693

• Detection Instrument:

  Microplate reader