Sphingosine 1 Phosphate Receptor 1 (EDG-1)

• Background:

  EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocyte- containing and other tissues.

• Synonyms:

  EDG1 (S1P1) Transfected Cell Positive Control Lysate

• Conjugation:

  Unconjugated

• Type:

  Control Lysate

• Applications:

  WB

• Field of Research:

  Ancillary Reagents

• Assay Principle:

  For use as a positive control with Exalpha's EDG1 (S1P1) polyclonal antibody (Cat. No. X1093P) for Western blot analysis.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided in 10% glycerol, 0,063 M Tris-HCl (pH 6,8), 2% SDS and 0,002% bromophenol blue, 5% 2-mercaptoethanol

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB