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CD4 Antibody

CAT: 0800-V9754-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V9754-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 55kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P01730
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 200-400 was used as the immunogen for the CD4 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
CD4/7144
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD4 antibody is available for research use only.
Storage Conditions
Aliquot the CD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CD4 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
IHC staining of FFPE human lymph node tissue with CD4 antibody (clone CD4/7144) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Product Datasheet
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