CD34 Antibody

• Description :

  This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intothree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidasebut sensitive to glycoprotease and chymopapain.CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt :

  P28906

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant full-length human HPCA1 protein was used as the immunogen for the CD34 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  CD34/4939

• Applications :

  IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Aliquot the CD34 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This CD34 antibody is available for research use only.

• Storage Conditions :

  Aliquot the CD34 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the CD34 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cell surface

• Image Legend :

  IHC staining of FFPE human placental tissue with CD34 antibody (clone CD34/4939) at 2ug/ml RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.