Transglutaminase 2 Antibody / TGM2

• Description:

  Recognizes a 77-85kDa protein, identified as cellular or tissue transglutaminase II (TGase II) . Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

• UniProt:

  P21980

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human Transglutaminase 2 protein was used as the immunogen for the Transglutaminase 2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  SPM358

• Applications:

  IHC-P, WB

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the Transglutaminase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Transglutaminase 2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Transglutaminase 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the Transglutaminase 2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell membrane, Cytoplasm, Secreted

• Image Legend:

  IHC staining of FFPE human placental tissue with Transglutaminase 2 antibody (clone SPM358) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.