AS3 Antibody / PDS5B

• Description:

  Sister chromatid cohesion protein PDS5 homolog B (PDS5B), also called Androgen-induced prostate proliferative shutoff-associated protein AS3, or simply 'AS3, ' is a protein that in humans is encoded by the PDS5B gene. This gene encodes a protein that interacts with the conserved protein complex termed cohesin. The cohesin complex holds together sister chromatids and facilitates accurate chromosome segregation during mitosis and meiosis. This protein is also a negative regulator of cell proliferation and may be a tumor-suppressor gene.

• Specifications:

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q9NTI5

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  E. coli-derived recombinant human protein (amino acids Q499-Q1424) was used as the immunogen for the AS3 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, Direct ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the AS3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This AS3 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the AS3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the AS3 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Flow cytometry testing of fixed and permeabilized human PC-3 cells with AS3 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= AS3 antibody.