Mouse Anti-Mouse PD-L1 (10F.9G2) Recombinant Antibody

Mouse Anti-Mouse PD-L1 (10F.9G2) Recombinant Antibody

• Background: CD274, also known as B7-H1 and PD-L1, is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes.
• Specifications: Mouse anti-mouse PD-L1 antibody (10F.9G2) is a recombinant antibody where the original rat IgG2b backbone of 10F.9G2 has been changed to Mouse IgG1. This antibody recognizes an epitope on Mouse CD274
• Product Name Alternative: Programmed cell death 1 ligand 1, Cd274, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
• CAS Number: 9000-83-3
• UniProt: Q9EP73
• Host: Mouse
• Species Reactivity: Mouse
• Target: PD-L1
• Isotype: IgG1
• Clone: 10F.9G2
• Applications: Flow Cytometry, Blocking, IHC (Frozen), Immunofluorescence, Functional Assays
• Purification Method: This monoclonal antibody was purified using Protein A
• Concentration: 1.0 - 5.0 mg/mL
• Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
• Purity: >95% by SDS-PAGE and HPLC
• Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
• Shipping Conditions: Blue ice
• Storage Conditions: Mouse Anti-mouse PD-L1 antibody (10F.9G2) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC.
• Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
• Antigen Distribution: The CD274 antigien is present on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes
• Aggregation: Aggregation level ≤ 5%
• Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
• Isotype Control: https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247