Mouse Anti-Mouse PD-L1 (10F.9G2) Recombinant Antibody

• Background:

  CD274, also known as B7-H1 and PD-L1, is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes.

• Specifications:

  Mouse anti-mouse PD-L1 antibody (10F.9G2) is a recombinant antibody where the original rat IgG2b backbone of 10F.9G2 has been changed to Mouse IgG1. This antibody recognizes an epitope on Mouse CD274

• Product Name Alternative:

  Programmed cell death 1 ligand 1, Cd274, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1

• CAS Number:

  9000-83-3

• UniProt:

  Q9EP73

• Host:

  Mouse

• Species Reactivity:

  Mouse

• Target:

  PD-L1

• Isotype:

  IgG1

• Clone:

  10F.9G2

• Applications:

  Flow Cytometry, Blocking, IHC (Frozen), Immunofluorescence, Functional Assays

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  1.0 - 5.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Mouse Anti-mouse PD-L1 antibody (10F.9G2) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  The CD274 antigien is present on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247