Anti-Mouse NKG2A/C/E (20D5) In Vivo Antibody - Low Endotoxin

Anti-Mouse NKG2A/C/E (20D5) In Vivo Antibody - Low Endotoxin

• Specifications: Clone 20D5 antibody reacts with mouse NKG2A, NKG2C, and NKG2E however, this hybridoma has not been epitope mapped to identify the binding site.
• Product Name Alternative: CD159a, CD159c, CD159e, NKG2C, NKG2E
• CAS Number: 9007-83-4
• Host: Rat
• Species Reactivity: Mouse
• Target: NKG2A/C/E
• Isotype: IgG2a
• Clone: 20D5
• Applications: Flow Cytometry
• Purification Method: This monoclonal antibody was purified using Protein G
• Concentration: 1.0 - 5.0 mg/mL
• Endotoxin: <1.0 EU/mg as determined by the LAL method
• Purity: >95% by SDS-PAGE and HPLC
• Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
• Shipping Conditions: Blue ice
• Storage Conditions: This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
• Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
• Antigen Distribution: NK cells and NK T cells from C57BL/6 mouse
• Aggregation: Aggregation level ≤ 5%
• Applications Notes: For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. An appropriate secondary used would be a goat-anti-mouse IgG2b FITC or brighter fluorochrome when needed.
• Isotype Control: www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244