Anti-Mouse NKG2A/C/E (20D5) In Vivo Antibody - Ultra-Low Endotoxin

• Specifications:

  Clone 20D5 antibody reacts with mouse NKG2A, NKG2C, and NKG2E however, this hybridoma has not been epitope mapped to identify the binding site.

• Product Name Alternative:

  CD159a, CD159c, CD159e, NKG2C, NKG2E

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  NKG2A/C/E

• Isotype:

  IgG2a

• Clone:

  20D5

• Applications:

  Flow Cytometry

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  1.0 - 5.0 mg/mL

• Endotoxin:

  ≤ 0.5 EU/mg as determined by the LAL method

• Purity:

  >98% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

• Antigen Distribution:

  NK cells and NK T cells from C57BL/6 mouse

• Aggregation:

  Aggregation level ≤ 1%

• Applications Notes:

  For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. An appropriate secondary used would be a goat-anti-mouse IgG2b FITC or brighter fluorochrome when needed.

• Isotype Control:

  www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

• CAS Number:

  9007-83-4