Anti-Mouse IFN Beta (MIB-5E9.1) In Vivo Antibody - Low Endotoxin

• Background: Interferons (IFN) consist of two classes. The type I IFN class consists of 20 highly similar IFN-alpha proteins and a single IFN-beta protein. The second class, type II IFN, consists of a single protein, IFN-gamma. IFN-alpha and IFN-beta signal through the same cell surface receptor IFNAR1 and have a similar range of biological activities including antiviral and antiproliferative activity. The type I IFNs also influence activation, growth and differentiation of T cells, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. IFN-beta has recently proven to be beneficial in patients with multiple sclerosis and could also be a potential therapy for rheumatoid arthritis. It is unclear what cellular mechanisms are involved in IFN-beta’s therapeutic effects, but they may be caused by a restoration of balance between pro- and anti-inflammatory cytokines.
• Specifications: Anti-IFN beta In Vivo Antibody - Low Endotoxin (MIB-5E9.1) recognizes Mouse IFN beta
• Product Name Alternative: IFN-b, IFN-beta, Type-1 interferon, Interferon beta
• CAS Number: 9007-83-4
• UniProt: P01575
• Host: Armenian Hamster
• Species Reactivity: Mouse
• Target: IFN beta
• Isotype: IgG
• Clone: MIB-5E9.1
• Applications: Western Blot, Blocking
• Purification Method: This monoclonal antibody was purified using Protein A
• Concentration: 1.0 mg/mL
• Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
• Purity: >95% by SDS-PAGE and HPLC
• Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
• Shipping Conditions: Blue ice
• Storage Conditions: Anti-IFN beta In Vivo Antibody - Low Endotoxin (MIB-5E9.1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
• Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
• Antigen Distribution: Fibroblasts & epithelial cells
• Aggregation: Aggregation level ≤ 5%
• Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
• Isotype Control: https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251