Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Low Endotoxin

Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Low Endotoxin

• Background: CD8b is also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes.
• Specifications: Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) recognizes an epitope on mouse CD8b.2 and antibody clone 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1
• Product Name Alternative: Cd8b, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3, Cd8b1, Ly-3, Lyt-3, Lyt3
• CAS Number: 9007-83-4
• UniProt: P10300
• Host: Rat
• Species Reactivity: Mouse
• Target: CD8 beta
• Isotype: IgG1
• Clone: 53-5.8
• Applications: Western Blot, Functional Assays, Flow Cytometry, Immunocytochemistry, IHC (Frozen), Immunoprecipitation
• Purification Method: This monoclonal antibody was purified using Protein G
• Concentration: 1.0 - 5.0 mg/mL
• Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
• Purity: >95% by SDS-PAGE and HPLC
• Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
• Shipping Conditions: Blue ice
• Storage Conditions: Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) is stable for at least one month when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
• Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
• Antigen Distribution: Majority of thymocytes, cytotoxic T cell subsets
• Aggregation: Aggregation level ≤ 5%
• Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
• Isotype Control: https://www.ichor.bio/product/Rat-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-GL113-ICH2246