Anti-Mouse Ly-6C (HK1.4) In Vivo Antibody - Low Endotoxin

• Specifications:

  Anti-Ly-6C In Vivo Antibody - Low Endotoxin (HK1.4) recognizes an epitope on Mouse Ly-6C

• Product Name Alternative:

  Lymphocyte antigen 6 complex, locus C

• UniProt:

  P0CW02

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  Ly-6C

• Isotype:

  IgG2c

• Clone:

  HK1.4

• Applications:

  Immunoprecipitation, Western Blot, Flow Cytometry, IHC (Frozen)

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  0.5 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-Ly-6C In Vivo Antibody - Low Endotoxin (HK1.4) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

• Antigen Distribution:

  The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Immunoprecipitation: 1–2 μg per 100–500 μg of total protein (1 ml of cell lysate). Each investigator should determine their own optimal working dilution for specific applications.

• CAS Number:

  9007-83-4