Anti-Mouse Ly-6G/Ly-6C (RB6-8C5) In Vivo Antibody - Low Endotoxin

• Background:

  Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.

• Specifications:

  Rat Anti-Mouse Granulocytes (Clone RB6-8C5) recognizes Mouse Granulocytes

• Product Name Alternative:

  Ly-6G/LY-6C, Gr-1, Myeloid

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  Ly-6G/Ly-6C

• Isotype:

  IgG2b

• Clone:

  RB6-8C5

• Applications:

  Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assays, IHC (Frozen), IHC (Paraffin), Immunocytochemistry, Immunofluorescence, In Situ Hybridization

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  0.5 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-Ly-6G/Ly-6C In Vivo Antibody - Low Endotoxin (RB6-8C5) is stable for at least four (4) week when stored at 2-8°C. For long-term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

• Antigen Distribution:

  The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

• CAS Number:

  9007-83-4