Anti-Mouse Ly-6G (1A8) In Vivo Antibody - Low Endotoxin

• Background:

  Ly-6G is a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

• Specifications:

  Anti-Ly-6G In Vivo Antibody - Low Endotoxin (1A8) recognizes an epitope on Mouse Ly-6G. Unlike clone RB6-8C5 antibody, the 1A8 antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C

• Product Name Alternative:

  Lymphocyte antigen 6 complex, locus G

• UniProt:

  P35461

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  Ly-6G

• Isotype:

  IgG2a

• Clone:

  1A8

• Applications:

  IHC (FFPE), IHC (Frozen), Flow Cytometry, Western Blot, In vivo Depletion, Immunfluorescence

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  1.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-Ly-6G In Vivo Antibody - Low Endotoxin (1A8) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

• CAS Number:

  9007-83-4