Anti-CD115 (AFS98) In Vivo Antibody - Low Endotoxin

• Background:

  Macrophage colony-stimulating factor (M-CSF, also termed CSF-1) receptor is an integral membrane tyrosine kinase, expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage. It also triggers growth and development of mononuclear phagocytes and placental trophoblasts. M-CSF receptor is present in osteoclasts both at mRNA and protein levels. When M-CSF ligand specifically binds to the M-CSF receptor on osteoclasts, it induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues M-CSF and its receptor present as candidate therapeutic targets in states of inflammatory bone erosion.

• Specifications:

  Anti-CD115 In Vivo Antibody - Low Endotoxin (AFS98) recognizes an epitope on Mouse M-CSFR

• Product Name Alternative:

  Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor (EC:2.7.10.1), CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms, Csf1r, Csfmr, Fms

• UniProt:

  P09581

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  CD115

• Isotype:

  IgG2a Kappa

• Clone:

  AFS98

• Applications:

  Western Blot, Blocking, Functional Assays, Flow Cytometry

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  1.0 - 5.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-CD115 In Vivo Antibody - Low Endotoxin (AFS98) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. and keep sterile.

• Formulation:

  0.01 M phosphate buffered saline (PBS), pH 7.2 - 7.4 without azide or BSA.

• Antigen Distribution:

  Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

• CAS Number:

  9007-83-4