Anti-Mouse CD54 (YN1/1.7.4) In Vivo Antibody - Low Endotoxin

• Background:

  Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.

• Specifications:

  Anti-CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4) recognizes an epitope on Mouse ICAM-1

• Product Name Alternative:

  Intercellular adhesion molecule 1, Icam1, MALA-2, MyD10

• UniProt:

  P13597

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  CD54

• Isotype:

  IgG2b Kappa

• Clone:

  YN1/1.7.4

• Applications:

  Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), Immunoprecipitation

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  1.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  The CD54 antigen is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. It has been demonstrated that ICAM-1 enhances T-cell activation.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

• CAS Number:

  9007-83-4