Anti-CD3e (145-2C11) In Vivo Antibody - Ultra-Low Endotoxin

• Background:

  CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

• Specifications:

  Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e

• Product Name Alternative:

  T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e

• UniProt:

  P22646

• Host:

  Armenian Hamster

• Species Reactivity:

  Mouse

• Target:

  CD3e

• Isotype:

  IgG

• Clone:

  145-2C11

• Applications:

  IHC (Frozen), Flow Cytometry, Immunoprecipitation, Western Blot, In vivo Depletion, Blocking

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  1.0 - 5.0 mg/mL

• Endotoxin:

  ≤ 0.5 EU/mg as determined by the LAL method

• Purity:

  >98% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin (145-2C11) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.

• Aggregation:

  Aggregation level ≤ 1%

• Applications Notes:

  Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251

• CAS Number:

  9007-83-4