Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin

• Background:

  CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex.

• Specifications:

  Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) recognizes an epitope on Mouse CD3ε

• Product Name Alternative:

  T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e

• UniProt:

  P22646

• Host:

  Syrian Hamster

• Species Reactivity:

  Mouse

• Target:

  CD3e

• Clone:

  500A2

• Applications:

  Functional Assays, Flow Cytometry, IHC (Frozen)

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  1.0 mg/mL

• Endotoxin:

  <1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Functional Assay: Binding of Hamster Anti-Mouse CD3ε (Clone 500 A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. Each investigator should determine their own optimal working dilution for specific applications.

• CAS Number:

  9007-83-4