Anti-ICAM-1 (15.2) In Vivo Antibody - Ultra-Low Endotoxin

• Background:

  Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.

• Specifications:

  Mouse anti-Human Inter-Cellular Adhesion Molecule 1 (ICAM-1) (Clone 15.2) - Ultra-Low Endotoxin recognizes an epitope on Human ICAM-1

• Product Name Alternative:

  Intercellular adhesion molecule 1, ICAM1, Major group rhinovirus receptor, CD54

• UniProt:

  P05362

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Target:

  ICAM-1

• Isotype:

  IgG1

• Clone:

  15.2

• Applications:

  IHC (FFPE), Flow Cytometry, Immunoprecipitation, Western Blot

• Purification Method:

  This monoclonal antibody was purified using Protein A

• Concentration:

  1.0 - 5.0 mg/mL

• Endotoxin:

  ≤ 0.5 EU/mg as determined by the LAL method

• Purity:

  >98% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

• Formulation:

  0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

• Antigen Distribution:

  The CD54 antigen is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes. CD54 is the receptor for rhinoviruses and malaria.

• Aggregation:

  Aggregation level ≤ 1%

• Applications Notes:

  Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247

• CAS Number:

  9007-83-4