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PAX5 Antibody / BSAP

CAT: 0800-RQ7931Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ7931Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Paired box protein Pax-5 is a protein that in humans is encoded by the PAX5 gene. The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q02548
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E. coli-derived recombinant human protein (amino acids Q166-K278) was used as the immunogen for the PAX5 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the PAX5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PAX5 antibody is available for research use only.
Storage Conditions
After reconstitution, the PAX5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PAX5 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC staining of FFPE human lymphoma tissue with PAX5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

UniProtKB · Q02548

Paired box protein Pax-5

PAX5_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q02548
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PAX5
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A3QVP6, A3QVP7, A3QVP8, C0KTF6, C0KTF7, C0KTF8, C0KTF9, C0KTG0, O75933, Q5SFM2, Q6S728, Q6S729, Q6S730, Q6S731, Q6S732
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Molecular function

Developmental proteinDNA-binding

Biological process

DifferentiationNeurogenesisSpermatogenesisTranscriptionTranscription regulation

Disease

Disease variantProto-oncogene

PTM

Glycoprotein

Cellular component

Nucleus

Domain

Paired box

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