Products for Research Use Only

Ki67 Antibody

CAT: 0800-RQ7913Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ7913Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P46013
Host
Mouse
Reactivity
Human
Immunogen
E. coli-derived recombinant human protein (amino acids K2860-I3256) was used as the immunogen for the Ki67 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
5C7
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Ki67 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Ki67 antibody is available for research use only.
Storage Conditions
After reconstitution, the Ki67 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Ki67 antibody should be determined by the researcher.
Location
Nucleus, Nucleolus
Image Legend
Immunofluorescent staining of FFPE human A431 cells with Ki67 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
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