RPA32 Antibody / Replication Protein A2

• Description:

  Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB) . RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively) . It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.

• Specifications:

  Western blot: 1-2 µg/mL

• UniProt:

  P15927

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Full length recombinant human protein was used as the immunogen for this RPA32 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  RPA2/2106

• Applications:

  WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the RPA32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This RPA32 antibody is available for research use only.

• Storage Conditions:

  Store the RPA32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  Western blot testing of human A375, K562 and MCF7 cell lysate with RPA32 antibody. Expected molecular weight ~32 kDa.