HACE1 Antibody

• Description:

  HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 is an E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. [UniProt]

• Specifications:

  Western blot: 1:2000

• UniProt:

  Q8IYU2

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human HACE1 was used as the immunogen for the HACE1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  1854CT757.66.47

• Applications:

  WB

• Purity:

  Protein G purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the HACE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This HACE1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the HACE1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the HACE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of HACE1 antibody at 1:2000: Lane 1) human HEK293 and 2) 293T/17 cell lysate. Predicted molecular weight ~102 kDa.