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Anti-ABCA1

CAT: 0013-GTR18086474Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18086474Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse monoclonal antibody to ABCA1
Reactivity
Human, Mouse, Other
Immunogen
Peptides amino acids 1800-2260 (C-Terminal end)
Clonality
Monoclonal
Clone
BCDXJ32
Conjugation
Unconjugated
Field of Research
Metabolism Research
Concentration
100ug/100ul
Dilution
Starting concentration for WB is 1:1000. This antibody has been tested with direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user.
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Starting concentration for WB is 1:1000. This antibody has been tested with direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user.
Tested Applications
ELISA, IHC, IP, WB
Host or Source
Mouse
Preservative
PBS, pH 7.4 with 0.05% sodium azide.
Isotype
IgG

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