CD116/CSF2RA Recombinant Protein

• Background:

  Granulocyte-macrophage colony-stimulating factor receptor subunit alpha (GM-CSF Receptor α), also known as CSF2RA and CD116, is a type 1 transmembrane protein with low affinity for GM-CSF. GM-CSF Receptor α is primarily expressed on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells. GM-CSF Receptor α forms a heterodimeric receptor complex with GM-CSF Receptor β, creating the high affinity GM-CSF receptor. Upon dimerization and ligand binding, the β subunit becomes phosphorylated and transduces signals via Jak/STAT and MAPK pathways for cell proliferation, differentiation, and survival. Oligomerization of the high affinity GM-CSF Receptor α/β complex is required for optimal intracellular signal transduction. Soluble GM-CSF Receptor α, either secreted or cleaved from the cell surface, can competitively bind GM-CSF, preventing membrane receptor signaling. Multiple isoforms of GM-CSF Receptor α have been described.

• Swiss Prot:

  P15509

• Modification Site:

  NdeI-XhoI

• Expression System:

  Pet-22b (+)

• Tag:

  His-tag

• Purity:

  Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

• Solubility:

  PBS, 4M Urea, PH7.4

• Molecular Weight:

  ~33kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Host or Source:

  E.coli

• CAS Number:

  9000-83-3

• AA Sequence:

  GAAAAGAGCGATCTGCGTACCGTGGCACCGGCAAGCAGCCTGAATGTTCGCTTCGATAGCCGCACCATGAATCTGAGCTGGGATTGCCAGGAAAATACCACCTTCAGTAAATGCTTCCTGACCGATAAAAAAAATCGTGTTGTGGAACCGCGCCTGAGTAATAATGAATGTAGCTGCACCTTCCGTGAAATCTGTCTGCATGAAGGCGTTACCTTCGAAGTGCATGTGAATACCAGCCAGCGTGGCTTCCAGCAGAAACTGCTGTATCCGAATAGTGGCCGTGAAGGTACCGCCGCACAGAACTTCAGTTGCTTCATCTATAATGCAGATCTGATGAATTGTACCTGGGCACGTGGTCCGACCGCACCGCGTGATGTGCAGTACTTCCTGTATATTCGCAATAGTAAACGCCGCCGCGAAATTCGCTGTCCGTATTATATTCAGGATAGTGGTACCCATGTGGGCTGTCATCTGGATAATCTGAGTGGTCTGACCAGTCGCAATTACTTCCTGGTTAATGGTACCAGTCGTGAAATTGGTATTCAGTTCTTCGATAGCCTGCTGGATACCAAAAAAATTGAACGCTTCAATCCGCCGAGTAATGTTACCGTGCGCTGCAATACCACCCATTGCCTGGTTCGTTGGAAACAGCCGCGCACCTATCAGAAACTGAGCTATCTGGACTTCCAGTATCAGCTGGATGTGCATCGTAAAAATACCCAGCCGGGTACCGAAAATCTGCTGATTAATGTTAGCGGTGATCTGGAAAATCGTTATAACTTCCCGAGTAGCGAACCGCGTGCAAAACATAGCGTGAAAATTCGTGCCGCAGATGTGCGTATTCTGAATTGGAGCAGTTGGAGTGAAGCAATTGAATTCGGTAGTGATGATGGC