CD109 Recombinant Protein

• Background:

  CD109 is a glycosylphosphatidylinositol (GPI) -linked glycoprotein that belongs to the alpha2-macroglobulin family of thioester containing proteins. CD109 is associated with TGF-beta receptor I (TbRI) and inhibits TGF-beta signaling. Cleavage of CD109 at its Furin cleavage site results in the release of its large amino-terminal domain, which then binds to the TGF-beta receptor I to inhibit TGF-beta signaling. CD109 is expressed on a subset of CD34+ bone marrow cells and mesenchymal stem cells, activated platelets, activated T cells, endothelial cells, and a wide variety of tumors. Elevated CD109 expression has been considered a diagnostic/prognostic marker for several types of cancers.

• Swiss Prot:

  Q6YHK3

• Modification Site:

  NdeI-XhoI

• Expression System:

  Pet-22b (+)

• Tag:

  His-tag

• Purity:

  Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

• Solubility:

  PBS, 4M Urea, PH7.4

• Molecular Weight:

  ~36kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Host or Source:

  E.coli

• CAS Number:

  9000-83-3

• AA Sequence:

  CTGAGCAGTGTGGGCAGCCCGAAAGCAAAAGAAGCACTGAATATGCTGACCTGGCGCGCAGAACAGGAAGGTGGCATGCAGTTCTGGGTGAGCAGTGAAAGCAAACTGAGCGATAGTTGGCAGCCGCGTAGCCTGGATATTGAAGTTGCAGCCTATGCACTGCTGAGTCACTTCCTGCAGTTCCAGACCAGCGAAGGTATTCCGATTATGCGTTGGCTGAGCCGTCAGCGCAATAGTCTGGGCGGCTTCGCAAGTACCCAGGATACCACCGTGGCCCTGAAAGCCCTGAGCGAATTCGCAGCACTGATGAATACCGAACGCACCAATATTCAGGTTACCGTGACCGGCCCGAGTAGCCCGAGCCCTGTGAAATTCCTGATTGATACCCATAATCGTCTGCTGCTGCAGACCGCAGAACTGGCAGTTGTGCAGCCGACCGCCGTTAATATTAGTGCCAATGGCTTCGGCTTCGCAATCTGTCAGCTGAATGTGGTGTATAATGTGAAAGCCAGTGGTAGCAGCCGCCGCCGCCGCAGCATTCAGAATCAGGAAGCATTCGATCTGGATGTGGCCGTTAAAGAAAATAAAGATGATCTGAATCACGTGGATCTGAATGTGTGTACCAGCTTCAGTGGCCCGGGTCGCAGTGGCATGGCACTGATGGAAGTTAATCTGCTGAGCGGCTTCATGGTTCCGAGTGAAGCAATTAGTCTGAGCGAAACCGTGAAAAAAGTTGAATATGATCATGGTAAGCTGAATCTGTATCTGGATAGTGTTAATGAAACACAATTCTGTGTGAATATCCCGGCAGTGCGTAACTTCAAAGTTAGCAATACCCAGGATGCAAGCGTTAGCATTGTGGATTATTATGAACCGCGCCGTCAGGCCGTGCGTAGCTATAATAGCGAAGTGAAACTGAGTAGTTGTGATCTGTGCAGTGATGTGCAGGGTTGTCGTCCGTGCGAAGATGGTGCA