NCK2 polyclonal antibody

• Background:

  This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  NCK2; GRB4; NCKbeta

• Swiss Prot:

  O43639

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human NCK2 (NP_003572.2) .

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC

• Dilution:

  WB,1:500 - 1:2000|IHC,1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  43kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Polyclonal Antibodies

• Applications Notes:

  Western blot analysis of extracts of various cell lines, using NCK2 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4