RBP2 polyclonal antibody

• Background :

  Rb (retinoblastoma protein) is a potent transcriptional regulator that is directly involved with events such as entry into cell division and formation of heterochromatin. RBP2 (Retinoblastoma-binding protein 2), also known as RBBP2, JARID1A (Jumonji/ARID domain-containing protein 1A) or KDM5A, is a nuclear protein that belongs to the JARID1 histone demethylase family. Expressed ubiquitously, RBP2 functions as a histone demethylase that, in conjunction with other proteins, binds directly to the viral-binding domain of Rb, thereby regulating Rb-mediated cell proliferation events. In addition, RBP2 can bind to the Rb-interacting protein rhombotin-2 (LMO2) and, through this interaction, can indirectly modulate Rb activity. Via its demethylase activity, RBP2 can remove methyl residues from Histone H3, thus playing a crucial role in the histone code. RBP2 contains one ARID domain, three PHD-type zinc fingers, one JMJN domain and one JMJC domain through which it conveys its enzymatic activity. Multiple isoforms of RBP2 exist due to alternative splicing events.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Retinol-binding protein 2; Cellular retinol-binding protein II; CRBP-II; RBP2; CRBP2

• Swiss Prot :

  P50120

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant full length Human RBP2.

• Conjugation :

  Unconjugated

• Applications :

  WB IHC IF

• Dilution :

  WB: 1:500~1:2000 IHC/IF: 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 16 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  RBP2 polyclonal antibody detects endogenous levels of RBP2 protein.

• Applications Notes :

  Western blot analysis of extracts of various cells, using RBP2 antibody.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4