NANOG polyclonal antibody

• Background:

  Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells. Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4. Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog; NANOG; Putative homeobox protein NANOG2; NANOGP1; NANOG2

• Swiss Prot:

  Q9H9S0/Q8N7R0

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding Human NANOG.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:1000~1:5000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 34 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  NANOG pAb detects endogenous levels of NANOG protein.

• Applications Notes:

  Western blot (WB) analysis of NANOG pAb at 1:2000 dilution Lane1:A2780 whole cell lysate (10ug) Lane2:Hela whole cell lysate (10ug) Lane3:3T3-L1 whole cell lysate (10ug) Lane4:The Testis tissue lysate of Rat (10ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4