EIF2S2 polyclonal antibody

• Background:

  The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF2 exists as a heterotrimeric complex of eIF2α, eIF2β and eIF2γ. eIF2 functions in the early stages of protein synthesis, by forming a ternary complex with GTP and tRNA. This complex binds to the 40S ribosomal subunit, followed by mRNA binding to 40S to form the 43S preinitiation complex, the release of eIF2 from 40S, and the hydrolysis of GTP. Phosphorylation of eIF2α correlates with inhibition of translation initiation.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Eukaryotic translation initiation factor 2 subunit 2; Eukaryotic translation initiation factor 2 subunit beta; eIF-2-beta; EIF2S2; EIF2B

• Swiss Prot:

  P20042

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 50-100 of Human EIF2S2.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 50 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  EIF2S2 polyclonal antibody detects endogenous levels of EIF2S2 protein.

• Applications Notes:

  Western blot (WB) analysis of EIF2S2 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4