EIF2B4 polyclonal antibody

• Background:

  The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF2B exists as a five subunit complex composed of eIF2Bα, eIF2Bβ, eIF2Bγ, eIF2Bδ and eIF2Bε. The eIF2B complex catalyzes the exchange of GDP for GTP on the eIF2 complex, following the interaction of eIF2/GTP with the 40S ribosomal subunit. Guanine nucleotide exchange factor (GEF) activity was exhibited by the eIF2Bε subunit alone, but it was greater in the presence of all five eIF2B subunits. Phosphorylation of eIF2 inhibits GEF activity of eIF2B, an inhibition that requires the eIF2Bα subunit.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Translation initiation factor eIF-2B subunit delta; eIF-2B GDP-GTP exchange factor subunit delta; EIF2B4; EIF2BD

• Swiss Prot:

  Q9UI10

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to Human EIF2B4.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB:1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 57 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  EIF2B4 polyclonal antibody detects endogenous levels of EIF2B4 protein.

• Applications Notes:

  Western blot (WB) analysis of EIF2B4 polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:MCF-7 whole cell lysate LaneC:PC12 whole cell lysate LaneD:CT26 whole cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4