EIF2B4 polyclonal antibody

CAT: 0384-BS62501-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS62501-01Size:50 µL
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Background
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF2B exists as a five subunit complex composed of eIF2Bα, eIF2Bβ, eIF2Bγ, eIF2Bδ and eIF2Bε. The eIF2B complex catalyzes the exchange of GDP for GTP on the eIF2 complex, following the interaction of eIF2/GTP with the 40S ribosomal subunit. Guanine nucleotide exchange factor (GEF) activity was exhibited by the eIF2Bε subunit alone, but it was greater in the presence of all five eIF2B subunits. Phosphorylation of eIF2 inhibits GEF activity of eIF2B, an inhibition that requires the eIF2Bα subunit.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Translation initiation factor eIF-2B subunit delta; eIF-2B GDP-GTP exchange factor subunit delta; EIF2B4; EIF2BD
Swiss Prot
Q9UI10
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to Human EIF2B4.
Conjugation
Unconjugated
Applications
WB
Dilution
WB:1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 57 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
EIF2B4 polyclonal antibody detects endogenous levels of EIF2B4 protein.
Applications Notes
Western blot (WB) analysis of EIF2B4 polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:MCF-7 whole cell lysate LaneC:PC12 whole cell lysate LaneD:CT26 whole cell lysate
Host or Source
Rabbit

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