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DDX54 polyclonal antibody

CAT: 0384-BS61585-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS61585-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX54 (DEAD polypeptide 54), also known as DP97, is an 881 amino acid protein that contains 2 bipartite nuclear localization signals, 3 nuclear receptor boxes (LXXLL motifs), a potential CoRNR box, and several stretches of glutamate and lysine residues. DDX54 is ubiquitously expressed, with highest expression in pancreas and lung. DDX54 co-localizes with ERα to structures in the nucleoplasm. DDX54 represses ERα transcriptional activity and acts as a nuclear receptor co-repressor against ERβ, progesterone, glucocorticoid and RARα
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
ATP-dependent RNA helicase DDX54 ; ATP-dependent RNA helicase DP97; DEAD box RNA helicase 97 kDa; DEAD box protein 54
Swiss Prot
Q8TDD1
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to amino acids 602-646 of Human DDX54
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Modification
Unmodification
Molecular Weight
~ 98 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
DDX54 polyclonal antibody detects endogenous levels of DDX54 protein.
Applications Notes
Western blot (WB) analysis of DDX54 polyclonal antibody at 1:500 dilution Lane1:HCC827 whole cell lysate (40ug) Lane2:L02 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit