CIDEB polyclonal antibody

• Background :

  The DNA fragmentation factor (DFF) is involved in the caspase-3 apoptotic pathway. DFF is composed of two subunits, a 45 kDa subunit called DFF-45 (also designated ICAD, for inhibitor of CAD), and CPAN (caspase-activated nuclease), also designated CAD (caspase-activated deoxyribonuclease) . CPAN is a DNase that is responsible for DNA degradation during apoptosis. CPAN is inhibited by DFF-45. Caspase-3 acts to dissociate CPAN from DFF-45, allowing CPAN to enter the nucleus and degrade DNA. CIDE-A and CIDE-B have been identified as proteins that share homology with the N-terminal region of DFF-45. Like CPAN, CIDE-A and CIDE-B promote cell death and DNA fragmentation and are inhibited by DFF-45.

• CAS Number :

  9007-83-4

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Cell death activator CIDE-B; Cell death-inducing DFFA-like effector B

• Swiss Prot :

  Q9UHD4

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids 104-148 of Human CIDEB

• Conjugation :

  Unconjugated

• Applications :

  WB

• Dilution :

  WB: 1:500~1:1000

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

• Modification :

  Unmodification

• Molecular Weight :

  ~ 28 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  CIDEB polyclonal antibody detects endogenous levels of CIDEB protein.

• Applications Notes :

  Western blot (WB) analysis of CIDEB polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:The Spleen lysate of Mouse (40ug) Lane4:The Spleen lysate of Rat (40ug)

• Host or Source :

  Rabbit