CIDEB polyclonal antibody

• Background:

  The DNA fragmentation factor (DFF) is involved in the caspase-3 apoptotic pathway. DFF is composed of two subunits, a 45 kDa subunit called DFF-45 (also designated ICAD, for inhibitor of CAD), and CPAN (caspase-activated nuclease), also designated CAD (caspase-activated deoxyribonuclease) . CPAN is a DNase that is responsible for DNA degradation during apoptosis. CPAN is inhibited by DFF-45. Caspase-3 acts to dissociate CPAN from DFF-45, allowing CPAN to enter the nucleus and degrade DNA. CIDE-A and CIDE-B have been identified as proteins that share homology with the N-terminal region of DFF-45. Like CPAN, CIDE-A and CIDE-B promote cell death and DNA fragmentation and are inhibited by DFF-45.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Cell death activator CIDE-B; Cell death-inducing DFFA-like effector B

• Swiss Prot:

  Q9UHD4

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 104-148 of Human CIDEB

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

• Modification:

  Unmodification

• Molecular Weight:

  ~ 28 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CIDEB polyclonal antibody detects endogenous levels of CIDEB protein.

• Applications Notes:

  Western blot (WB) analysis of CIDEB polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:The Spleen lysate of Mouse (40ug) Lane4:The Spleen lysate of Rat (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4