CD180 polyclonal antibody

• Background:

  RP105 is a mouse B-cell surface molecule that transmits a growth-promoting signal and is implicated in the life/death decision of B cells. RP105 has tandem repeats of a leucine-rich motif in its extracellular domain that are presumed to be involved in protein-protein interactions. The amino acid sequence of human RP105 is highly homologous to that of mouse RP105; human RP105 shares 74% identity with the mouse protein, as well as the leucine-rich motif. Surface expression of RP105 is enhanced in the presence of MD1, although this expression is restricted to CD19-positive B cells. RP105 demonstrates predominant expression on mature B cells in mantle zones; very little expression is observed in germinal centers.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  CD180 antigen; Lymphocyte antigen 64; Radioprotective 105 kDa protein; CD_antigen: CD180; LY64, RP105

• Swiss Prot:

  Q99467

• Reactivity:

  Human, Mouse

• Immunogen:

  A synthetic peptide corresponding to residues in Human CD180 .

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 74 kD

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD180 polyclonal antibody detects endogenous levels of CD180 protein.

• Applications Notes:

  Western blot (WB) analysis of CD180 polyclonal antibody at 1:1000 dilution Lane1:HEK293T whole cell lysate (57ug) Lane2:The spleen tissue lysate of Mouse (36ug) Lane3:RAW264.7 whole cell lysate (51ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4