CCP110 polyclonal antibody

Centrosome duplication and separation are linked inextricably to certain cell cycle events, specifically, activation of cyclin-dependent kinases of cyclin-dependent kinases. CP110 (centrosomal protein of 110 kDa) is a 991 amino acid cell cycle-dependent CDK substrate that regulates centrosome duplication. Localizing to the centrosome, CP110 contains ten putative CDK2 phosphorylation sites, two cyclin-binding domains and two degradation motifs. CP110 is highly expressed in testis with much lower expression in all other tissues. CP110 interacts with Ca2+-binding proteins including calmodulin (CaM) and centrin, to regulate genome stability and progression through cytokinesis. During the formation of cylindrical centrioles, it is suggested that CP110 acts as a distal end-capping protein thereby limiting the elongation of newly formed centrioles. Existing as two alternatively spliced isoforms, CP110 is observed at highest levels during the S phase of the cell cycle. CP110 becomes phosphorylated by Cdks (cyclin-dependent kinases) and is encoded by a gene located on human chromosome 16p12.3.

9007-83-4

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Centriolar coiled-coil protein of 110 kDa; Centrosomal protein of 110 kDa; CP110; Cep110; CCP110; CEP110; CP110; KIAA0419

O43303

Human

A synthetic peptide corresponding to residues in Human CCP110.

Unconjugated

WB

WB: 1:500~1:1000

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Unmodification

~ 130 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

CCP110 polyclonal antibody detects endogenous levels of CCP110 protein.

Western blot (WB) analysis of Centriolin (R899) pAb at 1:1000 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug)