NOTCH3 polyclonal antibody

• Background:

  The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. To date, four Notch homologs have been identified in mammals and have been designated Notch 1, Notch 2, Notch 3 and Notch 4. The Notch genes are expressed in a variety of tissues in both the embryonic and adult organism, suggesting that the genes are involved in multiple signaling pathways. The Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged, Jagged2 and Delta. Jagged can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. Jagged2 is thought to be involved in the development of various tissues whose development is dependent upon epithelial-mesenchymal interactions. Normal Delta expression is restricted to the adrenal gland and placenta. Delta expression has also been found in neuroendocrine tumors such as neuroblastomas and pheochromocytomas.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Neurogenic locus notch homolog protein 3; Notch 3; NOTCH3

• Swiss Prot:

  Q9UM47

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to residues in Human NOTCH3.

• Conjugation:

  Unconjugated

• Related Pathways:

  Notch Signaling

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 244 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  NOTCH3 polyclonal antibody detects endogenous levels of NOTCH3 protein.

• Applications Notes:

  Western blot (WB) analysis of NOTCH3 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4