NOTCH3 monoclonal antibody

• Background:

  Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate. Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) . Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes. Notch3 is a member of the Notch family and is processed similar to Notch1 It is expressed primarily in arterial smooth muscle cells (SMC) . Mutations altering the number of cysteine residues in the Notch3 extracellular region are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a hereditary angiopathy leading to strokes and dementia in adults. Recent studies indicate that Notch3 is overexpressed in many types of cancer.

• Structure Composition:

  Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  Neurogenic locus notch homolog protein 3; Notch 3

• Swiss Prot:

  Q9UM47

• Reactivity:

  Human

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NOTCH3. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Related Pathways:

  ESC Pluripotency and Differentiation Signaling Pathway, Notch Signaling

• Applications:

  WB

• Dilution:

  WB (1/500 - 1/1000)

• Purity:

  This antibody is purified through a protein G column.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 230 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of NOTCH3 protein.

• Applications Notes:

  Western blot analysis of NOTCH3 expression in Hela (A) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4